HAEMOGLOBIN ELECTROPHORESIS (HPLC) TEST
(Flat ₹201 OFF - Sanovra Lab)
Report Time: Same Day | Sample: Blood (EDTA) | Method: HPLC (Bio-Rad/Tosoh)
Haemoglobin HPLC (High Performance Liquid Chromatography) is the most accurate test available to detect abnormal types of haemoglobin in the blood. It is the gold standard for diagnosing genetic blood disorders such as Thalassemia (Minor & Major) and Sickle Cell Anemia.
This test separates and measures the different fractions of haemoglobin (HbA, HbA2, HbF, etc.) to identify hidden carriers of these genetic traits.
What is Thalassemia?
Thalassemia is a genetic disorder where the body makes abnormal haemoglobin, destroying red blood cells. It comes in two main forms:
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1.Thalassemia Minor (Trait/Carrier): These people are healthy carriers. They have mild anemia but live normal lives. However, if two carriers marry, their child has a 25% chance of being born with Thalassemia Major.
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2.Thalassemia Major: A severe disease requiring lifelong blood transfusions starting from infancy. Prevention (by testing parents) is the only cure.
Why is this Test Performed?
Doctors recommend Hb HPLC for:
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➤Antenatal Screening: Every pregnant woman should be tested to ensure the baby is not at risk.
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➤Pre-Marital Screening: Couples planning to marry should check if both are carriers.
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➤Unexplained Anemia: If your Haemoglobin is low but Iron supplements aren't helping, you might have Thalassemia Minor.
Interpreting Your Results
The HPLC report breaks down your haemoglobin into different Fractions:
| Haemoglobin Type | Normal Adult Range | Abnormal Indication |
|---|---|---|
| HbA (Adult) | > 95% | Decreased in Thalassemia. |
| HbA2 | 2.0% – 3.5% | > 3.5% indicates Beta Thalassemia Trait (Carrier). This is the key marker. |
| HbF (Fetal) | < 1.0% | Elevated in Thalassemia Major and HPFH (Hereditary Persistence of Fetal Hb). |
| HbS (Sickle) | 0% | Presence indicates Sickle Cell Trait or Disease. |
Frequently Asked Questions (FAQs)
No. Thalassemia Minor (Trait) is a genetic state. It stays the same for life and never turns into Major. The risk is only for your children if your partner is also a carrier.
No, fasting is not required for the HPLC test. You can eat and drink normally.
Yes. HPLC (High Performance Liquid Chromatography) is the modern, automated version. It is much more precise and sensitive than the older manual Electrophoresis method.
Secure Your Future Family
A simple blood test can prevent a lifetime of illness. Screen for Thalassemia with the Hb HPLC Test at Sanovra Lab.
Disclaimer: The information provided here is for educational purposes only and does not constitute medical advice. Laboratory results should always be interpreted by a qualified Hematologist or Gynecologist.